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KMID : 0370220000440050432
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Yakhak Hoeji
2000 Volume.44 No. 5 p.432 ~ p.439
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The Inhibitory Effects of Houttuynia cordata T_(HUNB) against Cadmium induced Cytotoxicity (Ⅱ)
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Lee Jeong-Ho
You Il-Soo
Kim Jong-Soo
Lee Ki-Nam
Jung Woo-Young
Han Du-Seok
Baek Seung-Hwa
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Abstract
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This study was conducted to investigate the antitoxic component in aqueous extract of Houttuynia cordata THUNB. The results were as follows: Generally, detoxification effects by Houttuynia cordata THUNB extract increased in proportion to the extract concentrations in rats. When 40mg/kg dosage of Houttuynia cordata THUNB extract was administrated, Houttuynia cordata THUNB extract showed the highest antitoxic effects in metallothionein induction. After the extract treatment, body weights increased in proportion to the extract concentrations. However, after 3 weeks, the body weight decreased insignificantly. From the above results, Houttuynia cordata THUNB extract increased metallothionein concentration and decreased the toxicity of cadmium in rats. In vitro the antitoxic activity of aqueous extract of Houttuynia cordata THUNB on NIH 3T3 fibroblasts was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide) and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows; The concentration of 10-2mg/ml of Houttuynia cordata THUNB extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were increased and. tend to regenerate. These results suggest that Houttuynia cordata THUNB extract retains a potential antitoxic activity.
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KEYWORD
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Houttuynia cordata T_(HUNB), Antitoxic agent, Metallothionein, MTT assay, SRB assay
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